Lab 3: Cell Growth Curve Adherent Cells

procedure

handwritten notes

Results

Hour 0-24 is the lag phase

Hour 48-96 is the log phase

hour 120-144 is the stationary phase

During the growth of the cells, our cells experienced a typical growth curve in which it starts out with an initial lag phase. On hour 48, the cells begun their exponential growth. Hour 72 did not have as much growth as expected but the cells rebounded by hour 96. As the cells went through hour 120, their growth begun to level out in the stationary phase. Based off the results of the growth curve, we can determine that our cells experienced a healthy cell cycle, although not perfect. The PDT (population doubling time) was estimated to be 20.62 which was taken during the log phase.

Conclusion

Based off the results, we can conclude that the experiment was successful. Our cells underwent a typical growth curve which included the lag phase, log phase, and stationary phase. We can determine that the cells are receiving the proper growth factors from the serum which is important in proliferation. Since we now have the PDT, we can use this information to determine doubling time for a cell line and plan in advance for subsequent cultures. Now that we have knowledge of cell growth, we can use this to our benefit. For example, we can manipulate the serum and add or take out our own reagents in it to see what causes cell growth to increase or decrease.

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