Lab 4: Cryopreservation of cells

Procedure

handwritten notes

Taking the cells out of the cryo storage container

Photo of me carefully taking the cells out of the cryo storage container to not get any liquid nitrogen on myself

Results

HEK 293 CELLS FLASK

Completed flask containing 0.5mL of cells and 10mL of MEM

Confluency

The confluency of the flask after 1 week was estimated to be at 70%

<-- cryocells flask

Flask containing the cryopreserved cells

<-- Cells post cryopreservation

The photo was taken two days after the cells were brought out of the cryo preservation container.

conclusion

In conclusion, the experiment provided valuable insight in cell preparation for cryopreservation and on the process of cryopreservation itself. The experiment was successful, reinforcing the already known findings in cryopreservation's importance. The cells used for this procedure can be split and used in future experiments.